The 5'-end expressed sequence tags of Lotus japonicus
نویسندگان
چکیده
منابع مشابه
Analysis of expressed sequence tags of flower buds in Lotus japonicus.
In order to study gene expression in a reproductive organ, we constructed a cDNA library of mature flower buds in Lotus japonicus, and characterized expressed sequence tags (ESTs) of 842 clones randomly selected. The EST sequences were clustered into 718 non-redundant groups. From BLAST and FASTA search analyses of both protein and DNA databases, 58.5% of the EST groups showed significant seque...
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For comprehensive analysis of genes expressed in a model legume, Lotus japonicus, a total of 22,983 5' end expressed sequence tags (ESTs) were accumulated from normalized and size-selected cDNA libraries constructed from young (2 weeks old) plants. The EST sequences were clustered into 7137 non-redundant groups. Similarity search against public non-redundant protein database indicated that 3302...
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Now a days, genome analysis has employed a rapid analysis tool known as expressed sequence tags. In 1983, SD Putney for the first time demonstrated the use of cDNA in identification of genome. The term expressed sequence tags (ESTs) was coined by Anthony Kerlavage at the Institute for Genomic Research. In 1991, Mark Adams used EST in relation to gene discovery and Human Genome project. cDNA or ...
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We have isolated a Lotus japonicus cDNA corresponding to a highly abundant, late nodule-specific RNA species that encodes a polypeptide with a predicted molecular mass of 15.6 kD. The protein and its corresponding gene were designated Nlj16 and LjNOD16, respectively. LjNOD16 was found to be expressed only in the infected cells of L. japonicus nodules. Related DNA sequences could be identified i...
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Supplementary Fig 1. Distribution of predicted proteins in ESTs of sacred lotus based on Gene Ontology classifications. (a) Cellular component; and (b) Biological process. The x-axis indicates the percentage of each category. 0 5 10 15 20 25 membrane intracellular cytoplasm nucleus plastid plasma membrane cytosol mitochondrion thylakoid endoplasmic reticulum Golgi apparatus vacuole extracellula...
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ژورنال
عنوان ژورنال: Plant Biotechnology
سال: 2008
ISSN: 1347-6114,1342-4580
DOI: 10.5511/plantbiotechnology.25.173